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1.
Environ Monit Assess ; 187(5): 278, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25893765

RESUMO

Increasingly, natural resource management agencies and nongovernmental organizations are sharing monitoring data across geographic and jurisdictional boundaries. Doing so improves their abilities to assess local-, regional-, and landscape-level environmental conditions, particularly status and trends, and to improve their ability to make short- and long-term management decisions. Status monitoring assesses the current condition of a population or environmental condition across an area. Monitoring for trends aims at monitoring changes in populations or environmental condition through time. We wrote this paper to inform agency and nongovernmental organization managers, analysts, and consultants regarding the kinds of environmental data that can be combined with suitable techniques and statistically aggregated for new assessments. By doing so, they can increase the (1) use of available data and (2) the validity and reliability of the assessments. Increased awareness of the difficulties inherent in combining and aggregating data for local- and regional-level analyses can increase the likelihood that future monitoring efforts will be modified and/or planned to accommodate data from multiple sources.


Assuntos
Monitoramento Ambiental/métodos , Conservação dos Recursos Naturais/métodos , Coleta de Dados , Poluição Ambiental/estatística & dados numéricos , Reprodutibilidade dos Testes
2.
Can Vet J ; 55(3): 240-8, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24587507

RESUMO

A total of 460 kidney samples from wildlife (beavers, coyotes, deer, foxes, opossums, otters, raccoons, skunks) were obtained from road-kill and hunter/trapper donations in Ontario between January 2010 and November 2012. The objectives of the study were to detect Leptospira spp. by immunohistochemistry and polymerase chain reaction (PCR), to map presence of leptospires in wildlife relative to livestock and human populations, and to characterize positive samples by sequencing and comparison to leptospires known to affect domestic animals and humans. The proportion of samples that tested positive ranged from 0% to 42%, with the highest rates in skunks and raccoons. Leptospira spp. were present in kidneys of wildlife across Ontario, particularly in areas of high human density, and areas in which livestock populations are abundant. The PCR was too weak in most samples to permit genotyping and examination of the relationship between the leptospires found in this study and those affecting domestic animals and humans.


Détection deLeptospiraspp. chez des hôtes du réservoir faunique en Ontario par la comparaison des méthodes de génotypage de réaction immunohistochimique et d'amplification en chaîne par la polymérase. Un total de 460 échantillons de reins provenant de la faune (castors, coyotes, cerfs de Virginie, renards, opossums, loutres, ratons-laveurs, moufettes) ont été obtenus d'animaux tués sur la route et de dons de chasseurs et de trappeurs en Ontario entre janvier 2010 et novembre 2012. Les objectifs de l'étude étaient de détecter Leptospira ssp. par immunohistochimie et amplification en chaîne par la polymérase afin de cartographier la présence des leptospires dans la faune en rapport avec les populations de bétail et d'humains et de caractériser les échantillons positifs par le séquençage et la comparaison avec des leptospires reconnus comme affectant les animaux domestiques et les humains. La proportion des échantillons qui ont montré un résultat positif s'échelonnait de 0 à 42 %, et les taux les plus élevés se retrouvaient chez les moufettes et les ratons-laveurs. Leptospira spp. était présent dans les reins de la faune partout en Ontario, particulièrement dans les régions à forte densité humaine et dans les régions où les populations de bétail sont abondantes. L'amplification en chaîne par la polymérase était trop faible dans la plupart des échantillons pour permettre le génotypage et l'examen de la relation entre les leptospires trouvés dans cette étude et ceux touchant les animaux domestiques et les humains.(Traduit par Isabelle Vallières).


Assuntos
Animais Selvagens , Leptospira/isolamento & purificação , Leptospirose/veterinária , Reação em Cadeia da Polimerase/veterinária , Animais , Reservatórios de Doenças/veterinária , Genótipo , Humanos , Imuno-Histoquímica , Leptospira/genética , Leptospirose/epidemiologia , Leptospirose/microbiologia , Gado , Ontário/epidemiologia , Reação em Cadeia da Polimerase/métodos
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